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Spécifications
- Description:Lactate Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Serum, plasma, cell culture media, etc.
- Processing time:20 minutes
- Cat. no.:75878-070
- Supplier No.:ECLC-100
Spécifications
A propos de cet article
For quantitative colorimertic determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
- Linear detection range 0.05 - 2 mM L-Lactate in 96-well plate assay
- Convenient room temperature assay. No 37°C heater is needed
- Can be readily automated to process thousands of samples per day
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready. Procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.