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Spécifications
- Description:Ascorbic Acid Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Serum, plasma, urine, saliva, milk, tissue, and cell culture.
- Processing time:10 minutes
- Cat. no.:75878-044
- Supplier No.:EASC-100
Spécifications
A propos de cet article
For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
- Linear detection range colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid
Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes
BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
By reacting with reactive oxygen species, it protects the cell from oxidative damages.