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Spécifications
- Description:Adipolysis Assay Kit
- Size:200 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Cell culture media
- Processing time:20 minutes
- Cat. no.:75878-042
- Supplier No.:EAPL-200
Spécifications
A propos de cet article
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
- Linear detection range 0.92 to 100 μg/mL (10 to 1000 μM) glycerol for colorimetric assays and 0.2 to 5 μg/mL for fluorimetric assays
- Robust and amenable to HTS assays
Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue
BioAssay Systems’ adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570 nm is directly proportional to glycerol concentration in the sample.
The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate. Procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.