To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Spécifications
- Description:Alanine Transaminase Assay Kit
- Size:100 assays
- Method:OD340 nm.
- Sample:Serum, plasma, etc.
- Cat. no.:75878-038
- Supplier No.:EALT-100
Spécifications
A propos de cet article
For quantitative determination of alanine transaminase activity.
- Linear detection range 2-100 U/L
- Assay can be carried out in a microplate or a cuvette and takes only 10 min
Alanine transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate
BioAssay Systems’ ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.
ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready. Procedures for measuring ALT activity find wide applications in research and drug discovery.