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Spécifications
- Description:Aconitase Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Biological samples (e.g. cell lysate, tissue homogenate, serum).
- Processing time:30 minutes
- Cat. no.:75878-032
- Supplier No.:EACO-100
Spécifications
A propos de cet article
For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
- Linear detection range 0.5 to 100 U/L for 20 min reaction
- Can be readily automated to process thousands of samples per day
Aconitase (Aconitate hydratase) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate
BioAssay Systems’ aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia and diabetes.