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Spécifications
- Description:Phosphate Assay Kit
- Size:500 assays
- Method:OD620 nm
- Sample:Serum, urine, saliva, sweat, tissue culture, food, environment etc.
- Processing time:30 minutes
- Cat. no.:75878-004
- Supplier No.:DIPI-500
Spécifications
A propos de cet article
For quantitative determination of phosphate concentrations.
- Linear detection range 0.3 - 50 μM (0.0028 - 0.47 mg/dL) phosphate in 96-well plate assay
- Can readily automated for processing thousands of samples per day
Phosphate (Pi) is one of the most important ion species in nature
BioAssay Systems’ phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring. Simple, direct and automation-ready. Procedures for measuring phosphate concentration in biological and environmental samples are becoming popular.