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Spécifications
- Description:Isocitrate Dehydrogenase Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Plasma, serum,tissue and culture media etc.
- Processing time:30 minutes
- Brand name:QuantiChrom™
- Cat. no.:75878-094
- Supplier No.:DIDH-100
Spécifications
A propos de cet article
For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism
- Fast and sensitive. Linear detection range (50µL sample): 0.5 µM to 60 µM acetaldehyde in 96-well plate assay.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay.
- Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
- Method: OD565nm
- Samples: Plasma, serum,tissue and culture media etc
ISOCITRATE DEHYDROGENASE (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. BioAssay Systems’ non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.