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Spécifications
- Description:Glucose-6-Phosphate Dehydrogenase Kit
- Size:100 assays
- Method:OD460 nm
- Sample:Plasma, serum, tissue and culture media, etc.
- Processing time:20 minutes
- Cat. no.:75877-978
- Supplier No.:DGPDH-100
Spécifications
A propos de cet article
For quantitative determination of glucose-6-phosphate dehydrogenase activity.
- Linear detection range 0.2 to 100 U/L
- Can be readily automated for processing thousands of samples per day
Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems’ non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.