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Spécifications
- Description:Glucose Dehydrogenase Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Biological samples (e.g. plasma, serum, tissue and culture media)
- Processing time:15 minutes
- Cat. no.:75877-972
- Supplier No.:DGDH-100
Spécifications
A propos de cet article
For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.
- Linear detection range 0.5 to 200 U/L for 15 min reaction
- Can be readily automated for processing thousands of samples per day
Glucose dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. BioAssay Systems’ non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.