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Spécifications
- Description:Glucosidase Assay Kit
- Size:100 assays
- Method:OD405 nm
- Sample:Biological
- Cat. no.:75877-952
- Supplier No.:DAGD-100
Spécifications
A propos de cet article
For quantitative determination of alpha-glucosidase activity.
- Linear detection range 2 to 250 U/L
- Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes
- All reagents are compatible with high-throughput liquid handling instruments
α-Glucosidase hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose
α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-D-glucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready. Procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems’ QuantiChrom™.