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Spécifications
- Description:Cell Viability Assay Kit
- Size:500 assays
- Method:OD570 nm
- Sample:Cell Culture
- Processing time:Five hours
- Cat. no.:75877-942
- Supplier No.:CQMT-500
Spécifications
A propos de cet article
Homogeneous colorimetric assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents.
- Non-radioactive assay (cf. 3H-thymidine incorporation assay)
- As low as 950 cells can be accurately quantified
- High-throughput assay using 96-well plates allows processing tens of thousands of samples per day
- No wash and reagent transfer steps are involved
The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis). The CellQuanti-MTT™ assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.