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Phenylalanine Agar is used for the differentiation of Enteric bacilli on the basis of their ability to produce phenylpyruvic acid by oxidative deamination.
- Phenylalanine serves as the substrate to deaminate
- Designed to differentiate members of Protease
- Dehydrated appearance: Light tan, free-flowing, homogeneous
The addition of 3 - 5 drops of a 10% aqueous ferric chloride solution (or a 12% aqueous ferric chloride solution acidified with 2.5 ml of concentrated HCl per 100 ml of reagent) to the cultures following incubation results in the appearance of a light to deep green color (positive reaction) or no color change (negative reaction).
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