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Spécifications
- Enzyme type:Recombinant
- Source:Cells
- Species:Human
- Size:0.05 mg
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme name:Sialate O-Acetylesterase
- Enzyme synonyms:Sialate O-Acetylesterase, H-Lse, Sialic Acid-Specific 9-O-Acetylesterase, SIAE, YSG2
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:57 kD
- Sequence:Ile24-Lys523
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10% Glycerol,pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
- Cat. no.:75790-118
- Supplier No.:91-729
Spécifications
A propos de cet article
Sialate O-Acetylesterase (SIAE) belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds
This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: C-6 His tag
SIAE is widely expressed with high expression levels in the testis, prostate, and colon. SIAE catalyzes N-acetyl-O-acetylneuraminate and H2O to N-acetylneuraminate and acetate. SIAE removes O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE down-regulates B lymphocyte antigen receptor signaling (involving CD22), and is required for immunological tolerance. Loss of function mutations in SIAE are much more frequently found in humans with autoimmune diseases especially rheumatoid arthritis and type 1 diabetes.