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Spécifications
- Enzyme type:Recombinant
- Source:Cells
- Species:Human
- Size:0.05 mg
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme name:Pyruvate Kinase
- Enzyme synonyms:Pyruvate Kinase Isozymes R/L, Pyruvate Kinase 1, R-Type/L-Type Pyruvate Kinase, Red Cell/Liver Pyruvate Kinase, PKLR, PK1, PKL
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:62.9 kD
- Sequence:Met1-Ser574
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM Tris-HCl, 1 mM DTT 0.2 M NaCl, 10% glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
- Cat. no.:75790-062
- Supplier No.:91-701
Spécifications
A propos de cet article
Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2
This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: C-6 His tag
L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.