Human Recombinant UBE2T (from E. coli)

# de catalogue 75789-050

Fournisseur: Prosci

Numéro CAS:

# de catalogue 75789-050

Fournisseur: Prosci

Numéro CAS:

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Spécifications

Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Size:
0.05 mg
Tag sequence:
N-6 His tag
Storage conditions:
Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Endotoxin content:
<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Protein synonyms:
Ubiquitin-Protein Ligase T|Ubiquitin-Conjugating Enzyme E2 T|UBE2T|Cell Proliferation-Inducing Gene 50 Protein|Ubiquitin Carrier Protein T|HSPC150
Protein/peptide name:
UBE2T
Purity:
> 95% as determined by reducing SDS-PAGE
Molecular weight:
24.7 kD
Sequence:
Met1-Val197
Formulation:
Supplied as a 0.2 um filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested applications:
Bioassay
Cat. no.:
75789-050
Supplier No.:
91-167

Spécifications

A propos de cet article

Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.