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Pierce™ Maleimide activated plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Available in clear for colorimetric assays, white for chemiluminescent assays and black for fluorescent assays.
- Pre-blocked to reduce nonspecific binding
- Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
- Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
- Activation Level: 100 µL
Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.
Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH> 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls.
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