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Spécifications
- Cell Format:Cryopreserved
- Description:Normal
- Size:0.1 mg
- Storage conditions:Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Storage buffer:Brain lysate (14 Days Old) is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Concentration:2 mg/mL
- Tested applications:WB
- Cat. no.:10062-386
- Source:Mouse
- Tissue:Brain
- Supplier No.:1403-14
Spécifications
A propos de cet article
Brain tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.