NELL2 is a neuron-specific thrombospondin-1-like extracellular protein containing six epidermal growth factor-like domains (1). It is highly expressed in the hippocampus in vivo (2) and promotes survival of rat primary cultured neurons through activation of JNK and suppression of ERK (3). As ERK is required for the induction of hippocampal long term potentiation (LTP) and JNK appears to be inhibitory on LTP (4,5), it had been suggested that NELL2 may play a role in synaptic plasticity. More recently, NELL2 has been shown to promote differentiation of motor and sensory neurons and stimulates mitogenesis of dorsal root ganglia in chickens (6).

This recombinant protein can be used for WB, ELISA, MS and neutralization assays.

100mM sodium phosphate, 10mM Tris, 500mM NaCl, 25 mM imidazole, 2mM MgCl2, 10% gycerol

Store in working aliquots at -80°C. Avoid freeze/thaw cycles.