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Spécifications
- Cell Format:Cryopreserved
- Description:Normal
- Size:0.1 mg
- Storage conditions:Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Storage buffer:Adipose lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Concentration:2 mg/mL
- Tested applications:WB
- Cat. no.:10062-280
- Source:Rat
- Tissue:Adipose
- Supplier No.:1477
Spécifications
A propos de cet article
Adipose tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors)
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.