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Spécifications
- Antibody type:Primary
- Antigen name:bone morphogenetic protein 4
- Clonality:Monoclonal
- Clone:3H2
- Gene ID:P12644
- Host:Mouse
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:BMP4
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.5 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:BMP4
- Shipping temperature:RT
- Immunogen:BMP-4 antibody is produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen.
- Purification:This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
- Cat. no.:10097-918
- Supplier No.:38-231
Spécifications
A propos de cet article
BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 µg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 µL/well), a concentration of 4.0-8.0 µg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
Type: Primary
Antigen: BMP4
Clonality: Monoclonal
Clone: 3H2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human