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Spécifications
- Antibody type:Primary
- Antigen name:ubiquitin-like modifier activating enzyme 3
- Clonality:Polyclonal
- Gene ID:Q8TBC4
- Host:Rabbit
- Reactivity:Human,Rat,Mouse
- Antigen symbol:UBA3
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 ul
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:UBA3
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:52 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store UBA3 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBA3.
- Purification:Antibody is purified by peptide affinity chromatography method.
- Cat. no.:10111-564
- Supplier No.:31-292
Spécifications
A propos de cet article
UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UBA3 antibody can be used for detection of UBA3 by ELISA at 1:1562500. UBA3 antibody can be used for detection of UBA3 by western blot at 1 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: UBA3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat