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Spécifications
- Antibody type:Primary
- Antigen name:general transcription factor IIE, polypeptide 1, alpha 56kDa
- Clonality:Polyclonal
- Gene ID:P29083
- Host:Rabbit
- Reactivity:Human,Dog,Rat,Mouse
- Antigen symbol:GTF2E1
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- Size:100 ul
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:GTF2E1
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:49 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store GTF2E1 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1.
- Purification:Antibody is purified by peptide affinity chromatography method.
- Cat. no.:10111-350
- Supplier No.:31-216
Spécifications
A propos de cet article
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: GTF2E1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat, Dog