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Spécifications
- Description:NEDDylation Kit
- Size:1 Kit
- Storage temperature:-80 °C
- Cat. no.:89165-182
- No. of Reactions:20
- Supplier No.:UW0590-0001
Spécifications
A propos de cet article
This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied)
- This kit provides sufficient material for 20 x 20µL reactions
- Generate NEDD8-E2 thioesters for use in the in vitro NEDDylation of target proteins
- Demonstrate novel proteins are potential targets for NEDDylation
- Study NEDD8 activation (E1) and conjugation (E2) steps in the NEDDylation cascade
- Generate substrates for deNEDDylating enzymes
- Test proteins for NEDD8 E3 ligase activity
- Avoid freeze/thaw cycles
Note: This Kit does not contain any NEDD8 E3 ligases.
A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.