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Spécifications
- Size:0.5 µmol
- Protein/peptide name:FLUOR DE LYS®-Sirt1 Deacetylase Substrate
- Purity:>95% (HPLC)
- Formulation:Supplied as a 5 mM solution in HDAC Assay Buffer.
- Cat. no.:89160-346
- Supplier No.:KI177-0005
Spécifications
A propos de cet article
Fluor de Lys®-SIRT1 is a fluorogenic, acetylated peptide substrate for SIRT1 (human Sirtuin 1). Based on residues 379-382 of p53 (Arg-His-Lys-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases (lysines 381, 382), it was the best substrate for SIRT1 from among a panel of substrates patterned on p53, histone H3, and histone H4 acetylation sites. Fluor de Lys®-SIRT1 is deacetylated by SIRT1 (BML-SE239) at a rate of more then 8-fold that of the acetylated lysine substrate, Fluor de Lys® (Prod. No. BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). The Km of Fluor de Lys®-SIRT1 for human recombinant Sirtuin 1 is 108 µM (determined at 37°C, 500 µM NAD+). Must be used in conjunction with Fluor de Lys® Developer II (Prod. No. BML-KI176). Fluorescent signal indicates deacetylation of Lys382. Sufficient for 100-200 assays of human recombinant SIRT1 (1U/well, 50-100 µM substrate).