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Spécifications
- Antibody type:Primary
- Antigen name:Harvey Rat Sarcoma Viral Oncogene Homolog
- Clonality:Monoclonal
- Clone:Y13-259
- Gene ID:3265
- Host:Rat
- Isotype:IgG
- Reactivity:Human
- Antigen symbol:VH RAS
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:C-H-RAS|CTLO|p21ras|HRAS1|RASH1|C-HA-RAS1|H-RASIDX|HAMSV|C-BAS/HAS
- Storage buffer:Preservative: 0.1% Sodium Azide; Constituents: 0.2% gelatin, 0.05M Sodium Phosphate. pH 7.5
- Concentration:0.1 mg/ml
- Immunogen:Recombinant full length p21 protein (Human).
- Tested applications:ICC/IF, IHC-P, IHC-Fr, IP, WB
- Purification:Protein G purified
- Cat. no.:89350-864
- Supplier No.:GTX13056
Spécifications
A propos de cet article
RAS proteins are signal-transducing, guanine nucleotide-binding proteins that appear to function as a branchpoint in signal transduction. RAS coordinates the activity of multiple signalling pathways, regulating diverse cellular functions including cell growth, differentiation and apoptosis. The human RAS gene family consists of three identified members which encode proteins of 21 kDa. Human cH RAS and cK RAS are the cellular homologs of vH- and vK RAS originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. The third family member is designated cN RAS. Normal cellular ras genes are referred to as protooncogenes and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61. Such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis remains to be determined.
IF: Use at a concentration of 2.5 ?g/ml. IHC-P: Use at a concentration of 5 ?g/ml. Perform enzymatic antigen retrieval (0.05% saponin in water, 30 min. or 0.1% pepsin in 0.1 M HCl, 10-20 min. at room temperature) before commencing with IHC staining protocol. IHC-Fr: Use at a concentration of 5 ?g/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2.5 ?g/ml. Predicted molecular weight: 21 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: VH RAS
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rat
Isotype: IgG
Reactivity: Human