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Spécifications
- Antibody type:Primary
- Antigen name:Perforin
- Clonality:Monoclonal
- Clone:CE2.10
- Gene ID:18646
- Host:Mouse
- Isotype:IgG2a
- Reactivity:Human
- Antigen symbol:PRF1
- Conjugation:Unconjugated
- ImmunoPrecipitation:Yes
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:lymphocyte pore-forming protein|cytolysin|lymphocyte pore forming protein|perforin-1
- Storage buffer:Preservative: 0.02% Sodium Azide; Constituents: PBS
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.5 mg/ml
- Immunogen:Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.
- Tested applications:IP, WB
- Purification:>95% by SDS-PAGE
- Cat. no.:89360-146
- Supplier No.:GTX16079
Spécifications
A propos de cet article
Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T-cell or NK-cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin (PRF) is involved in the killing mediated by cytotoxic lymphocytes which represents an important mechanism in the immune defense against tumors and virus infections. Human PRF is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kD. PRF is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.
IP: Use at an assay dependent dilution on human and mouse samples. WB: Use at a concentration of 1 ?g/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa) from the mouse T cell clone B6.1. The antibody does not detect human perforin by Western blot. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: PRF1
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human