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Spécifications
- Antibody type:Primary
- Antigen name:Interleukin 8
- Clonality:Monoclonal
- Clone:6217
- Gene ID:3576
- Host:Mouse
- Isotype:IgG1
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:IL8
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:MDNCF|Interleukin-8|T-cell chemotactic factor|LYNAP|NAP1|NAP-1|GCP1|Monocyte-derived neutrophil chemotactic factor|MONAP|LECT|NAF|GCP-1|CXCL8|LUCT|Neutrophil-activating protein 1
- Storage buffer:Preservative: NoneConstituents: PBS containing 5% Trehalose.
- Storage temperature:Keep as concentrated solution. Aliquot and store at –20 °C or below. Avoid multiple freeze-thaw cycles.
- Concentration:0.5mg/ml
- Immunogen:Recombinant full length protein (Human).
- Tested applications:Fluorescence-Activated Cell Sorting
- Purification:Protein A purified
- Cat. no.:89355-058
- Supplier No.:GTX10768
Spécifications
A propos de cet article
Mouse monoclonal [6217] to IL8
ELISA: Use at a concentration of 4 ?g/ml. FACS: Use at a concentration of 25 ?g/ml. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 ?g/ml. Predicted molecular weight: 11 kDa. Monoclonal Anti-Interleukin 8 has the ability to neutralize human IL8 bioactivity. The ND50 for this effect is approximately 0.08-0.4?g/ml in the presence of 0.02?g/ml of recombinant human IL8 using hCXCR-2 transfected BaF/3 cells. The exact concentration of antibody required to neutralize recombinant human IL8 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity. The detection limit for in immunoblotting for recombinant human IL8 is approximately 0.5 ng/lane under non-reducing and reducing conditions in SDS PAGE. For capture ELISAs, monoclonal anti-interleukin 8 can be used as the capture antibody. A working concentration of the capture antibody at 4?g/ml (100?l/well) in combination with a biotinylated detection antibody at 20 ng/ml (100?l/well) is recommended to detect human IL8. For flow cytometry (intracellular staining), the cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. The cells (100,000) are stained with the primary antibody using 10?l of a 25?g/ml stock solution. Following a 30 minute incubation, the cells should be washed with 0.1% saponin prior to using a fluorochrome conjugated secondary reagent for detection (typically, 250 ng of a polyclonal antibody per reaction is added to develop the staining). After incubation with the fluorochrome, the cells should be washed for a final time in 0.1% saponin prior to analysis. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: IL8
Clonality: Monoclonal
Clone: 6217
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human