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Spécifications
- Antibody type:Primary
- Antigen name:Cyclin-dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4)
- Clonality:Polyclonal
- Gene ID:1029
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human
- Antigen symbol:p14ARF
- Conjugation:Unconjugated
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:100 μl
- Western blot:Yes
- Antigen synonyms:CDKN2|MLM|INK4|p16INK4a|p16|p16INK4|p19|p14|p19Arf|CDK4I|INK4a|p14ARF|CMM2|ARF|MTS1|TP16
- Storage buffer:PBS and 0.05% Sodium Azide as a preservatives
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:A synthetic peptide made to a portion of human p14ARF (between residues 50-150) .
- Tested applications:ICC/IF, WB
- Purification:Immunogen affinity purified
- Cat. no.:89367-524
- Supplier No.:GTX20470
Spécifications
A propos de cet article
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
We recommend the following starting dilutions:
For WB: Use at a dilution of 1:100-1:2000.
For FACS: Use at a dilution of 1:400.
For ELISA: Use at a dilution of 1:100-1:2000.
For ICC/IF: Use at a dilution of 1:50-1:200.
For IHC, IHC-Fr, IHC-P: Use at a dilution of 1:10-1:500.
For IP: Use at an assay dependent dilution.
Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: p14ARF
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human