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Spécifications
- Antibody type:Primary
- Antigen name:Matrix Metalloproteinase 14
- Clonality:Polyclonal
- Gene ID:4323
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human
- Antigen symbol:MMP14
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:EC 3.4.24.80|Membrane-type-1 matrix metalloproteinase|MTMMP1|Matrix metalloproteinase-14|MMP-X1|MT1MMP|MT1-MMP|Membrane-type matrix metalloproteinase 1|MT-MMP 1|MMP-14|MMP14
- Storage buffer:Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.25 mg/ml
- Immunogen:Synthetic peptide (Human) N-term.
- Tested applications:ELISA, IHC-P, WB
- Purification:Immunogen affinity purified
- Cat. no.:89358-584
- Supplier No.:GTX11621
Spécifications
A propos de cet article
The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimers, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 65 (pro-form) and 63 kDa (active form). MMP14 (MT1MMP) is elevated in several tumor cell lines and is constitutively produced by some normal cell lines. Treatment of cells with concanavalin A or the phorbal ester TPA stimulates production of MT1MMP in some cell types. The enzyme can be recovered in cell lysates. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: MMP14
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human