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Spécifications
- Antibody type:Primary
- Antigen name:AKT
- Clonality:Polyclonal
- Gene ID:207
- Host:Chicken
- Isotype:IgY
- Reactivity:Bovine
- Antigen symbol:AKT
- Conjugation:Unconjugated
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:CWS6|RAC|RAC-ALPHA|PRKBA|PKB|AKT|PKB-ALPHA
- Storage buffer:PBS, no preservatives added
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1.0 mg/ml
- Immunogen:Recombinant fragment: SDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR, corresponding
- Tested applications:WB
- Purification:Affinity purified with antigen
- Cat. no.:89367-214
- Supplier No.:GTX13990
Spécifications
A propos de cet article
AKT (Protein Kinase B, PKB) was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
For WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: AKT
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Chicken
Isotype: IgY
Reactivity: Bovine