You searched for: Cloning Kits and Ligation
The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
BioXp® Select DNA Cloning Gibson Assembly Kits
Supplier: TELESIS BIO
The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.
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BIOXP® 9600 Select Plasmid AMP
Supplier: TELESIS BIO
BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.
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BioXp® Select DNA Cloning and Amplification Kits
Supplier: TELESIS BIO
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.
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Gibson Assembly® ULTRA Kits and Master Mixes (2X)
Supplier: TELESIS BIO
Generate seamless plasmid DNA or BAC clones without restriction digestion.
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BioXp® Select DNA Cloning Golden Gate Assembly BsmBI Kits
Supplier: TELESIS BIO
The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.
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BIOXP® 9600 Select Clone and AMP
Supplier: TELESIS BIO
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.
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Gibson Assembly® HIFI 1-Step Kits and Master Mixes (2X)
Supplier: TELESIS BIO
Generate seamless plasmid DNA or BAC clones without restriction digestion.
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BIOXP® Select Plasmid AMP
Supplier: TELESIS BIO
BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.
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BioXp® Select DNA Cloning Golden Gate Assembly, BsaI Kits
Supplier: TELESIS BIO
The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.
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Seamless Assembly Master Mix (2X)
Supplier: ReVacc Scientific
This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.