You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
1X TBS pH 7.8
Supplier: Rockland Immunochemical
1X Tris buffered saline (TBS) pH 7.8
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RNA Gel Loading Solution 10X, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989)
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TBS 10X Buffer Solution, pH 7.4, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
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10X PBS pH 7.2
Supplier: Rockland Immunochemical
10X Phosphate buffered saline (PBS) pH 7.2
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1X PBS Buffer with 1% Casein
Supplier: Rockland Immunochemical
Phosphate buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.
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SSPE Buffer, 20X, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.
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SSC, 20X Buffer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization
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20X SSC Buffer (Standard Sodium Citrate)
Supplier: GROWCELLS
Concentrated sodium chloride-sodium citrate buffer functions by providing high ionic strength to optimize the rate of probe annealing to target nucleic acid
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Blocking Solution, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.
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Electrophoresis Running Buffer
Supplier: Edvotek
Tris Acetate, pH 7.8, concentrated 50X (for 5 L—20 standard gels).
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TBE Buffer Solution
Supplier: Biotium
5X TBE for preparation of agarose gel running buffer.
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PreMixed Running Buffers, IBI Scientific
Supplier: IBI Scientific
IBI provides a variety of premixed buffers in various configurations
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Tris-Buffered Saline with Tween Solution (10X), Genetex
Supplier: Genetex
Dilute this 10X buffer 10 times, mix well, and store 1 X buffer solution at room temperature or 2-8oC
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Phosphate Buffered Saline, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
PBS is commonly used in biochemistry.
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Phosphate Buffered Saline (PBS) 20X, Genetex
Supplier: Genetex
This solution is sufficient to prepare 10 L of 1X phosphate buffered saline
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PBS Phosphate Buffered Saline Tablets, Genetex
Supplier: Genetex
Pre-measured Phosphate Buffered Saline Tablets
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Dry Milk Powder, Biotium
Supplier: Biotium
Milk is commonly used in blocking buffers minimize background noise in immunodetection procedures such as Western Blotting and immunostaining
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TrueBlack® WB Blocking Buffer Kit, Biotium
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).