You searched for: Nucleotides
Gold 550 dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Fluorescein-12-ddUTP dideoxynucleotide pack for 3'-oligonucleotide labeling, Enzo Life Sciences
Supplier: Enzo Life Sciences
The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions.
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Red 650 dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Cyanine 3-dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.
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Cyanine 5-dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
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VWR® dNTPs
Supplier: VWR International
Ready to use molecular biology grade dNTP mixes and dNTP sets.
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Green 496 dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Allylamine-dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Allylamine-2’-deoxyuridine-5’-triphosphate can replace TTP in reactions where it serves as a substrate for E. coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).
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Bio-16-ddUTP . tetralithium salt, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes.
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Bio-7-dATP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-7-dATP (Biotin-7-2’-deoxyadenosine-5’-triphosphate) can replace dATP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Bio-11-CTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Bio-16-UTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Bio-N6-ddATP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-N6-ddATP (Biotin-N6-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N6-ddAMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes.
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dNTP Mix, Zymo Research
Supplier: Zymo Research
The dNTP Mix, along with dATP, dTTP, dGTP, and dCTP mixes from Zymo Research are of ultra-high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
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Fluorescein-12-UTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.
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Fluorescein-12-ddUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.
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Cyanine 3-UTP (enhanced), Enzo Life Sciences
Supplier: Enzo Life Sciences
Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
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Cyanine 5-UTP (enhanced), Enzo Life Sciences
Supplier: Enzo Life Sciences
Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.
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Bio-16-dUTP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Bio-17-ATP, Enzo Life Sciences
Supplier: Enzo Life Sciences
Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis.
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dNTP Set, Biotium
Supplier: Biotium
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mm.
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Digoxigenin-dUTP, Alkali Stable, Biotium
Supplier: Biotium
Digoxigenin-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3'-end terminal labeling to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. The digoxigenin (DIG) labeled probe could be detected using a labeled anti-digoxigenin antibody.
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5-Tetramethylrhodamine-dUTP, 1 mM Solution, Biotium
Supplier: Biotium
5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA to make fluorescently labeled probes, and has absorption/emission at 553/577 nm. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris-HCl buffer, pH 7.5.
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dNTP Mix, Biotium
Supplier: Biotium
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.