5073 Results for: "that (Tris-(succinimidyl)aminotriacetate)"

Supplier: LONZA PHARMA - BIOSCIENCE CA

This is a neural cell specific basic Nucleofector® kit which allows to determine the optimal transfection conditions for any mammalian neuron type. In contrast to our standard basic kit (VPI-1003) this kit is suited for transfection of small cell numbers.

Supplier: Prosci

NELL2 is a neuron-specific thrombospondin-1-like extracellular protein containing six epidermal growth factor-like domains (1). It is highly expressed in the hippocampus in vivo (2) and promotes survival of rat primary cultured neurons through activation of JNK and suppression of ERK (3). As ERK is required for the induction of hippocampal long term potentiation (LTP) and JNK appears to be inhibitory on LTP (4,5), it had been suggested that NELL2 may play a role in synaptic plasticity. More recently, NELL2 has been shown to promote differentiation of motor and sensory neurons and stimulates mitogenesis of dorsal root ganglia in chickens (6).

Supplier: Prosci

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) (1). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).

Supplier: Thermo Scientific

Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

Supplier: G-Biosciences

G-Biosciences' recombinant Taq Polymerase is a highly thermostable DNA polymerase isolated from the thermophile, Thermus aquaticus

Supplier: G-Biosciences

G-Biosciences' Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase

Supplier: Prosci

Polyclonal antibody LRP5 Host: Goat Target Species: human immunogen: LRP5 antibody was raised against a 12 amino acid synthetic peptide near the internal region of LRP5. Application: ELISA, WB, IF

Supplier: LONZA PHARMA - BIOSCIENCE CA

The cell line optimization 96-well Nucleofector® kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.

Supplier: Prosci

NHP2-Like Protein 1 (NHP2L1) is a member of the ribosomal protein L7Ae family. NHP2L1 proteinis limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 has been shown to interact with RAD17and PRPF31. The protein undergoes a conformational change upon RNA-binding. NHP2L1 binds to the 5-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly, prior to step I of splicing catalysis.

Supplier: Thermo Scientific

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10kDa to 200kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: Thermo Scientific

Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small-protein gel electrophoresis.

Supplier: Thermo Scientific

The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.

Supplier: Cytiva

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

Supplier: Thermo Scientific

Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5kDa to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: LONZA PHARMA - BIOSCIENCE CA

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-Nucleofector® Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state.

Supplier: CUBE BIOTECH

DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Supplier: Prosci

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, α, β and γ have been identified. The α actins are found in muscle tissues and are a major constituent of the contractile apparatus. The β and γ actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, γ 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Supplier: Prosci

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).

Supplier: LONZA PHARMA - BIOSCIENCE CA

The P3 Primary Cell 96-well Nucleofector® kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well unit.

Supplier: Binder

The CBF series CO₂ incubators are the premium series of CO₂ incubators with humidity control. Units are suitable for all sensitive incubation applications and ensure optimal cell growth in GMP environments. A CBF series CO₂ incubator can be used for complex cultivation experiments or individual environments with hypoxic conditions thanks to the comprehensive range of options and accessories. Units ensure consistent results including in cell-based assays with multiwell plates.

Supplier: Prosci

Nuclear factor kappa B (NF-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).

Supplier: Prosci

Cyclin-dependent kinase 2 (CDK2), a CDC2/CDK-type protein kinase, regulates cell-cycle transition from G1 to S phase. CDK2 activation requires formation of a heterodimer with cyclin A, D, or E and phosphorylation by CAK. CDK2 inhibition results from association with p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Two alternatively spliced variants and multiple transcription initiation sites have been reported. Inhibition of CDK2 has been suggested to help prevent chemotherapy-induced alopecia in cancer patients.

Supplier: Genetex

PE-conjugated Mouse Monoclonal antibody [B2M-01] to beta2-microglobulin

Supplier: LONZA PHARMA - BIOSCIENCE CA

The cell line optimization 4D-Nucleofector® X kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® X unit.

Supplier: Enzo Life Sciences

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

Supplier: Nidec Shimpo America

The Series DT-2100 Data-Logging Tachometer is the most highly advanced tachometer ever developed by NIDEC-SHIMPO.

Supplier: Biosensis

Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).

Supplier: Thermo Scientific

The Thermo Scientific Pierce™ classic IP kit uses high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays with ease.

Supplier: LONZA PHARMA - BIOSCIENCE CA

The Nucleofector® 2b device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector® kit 1 is one of our two kits suited for efficient transfection of human stem cells.

Supplier: Thermo Scientific

These Glutathione spin columns provide a fast, easy-to-use format to purify GST-fusion proteins from cellular lysates.