22646 Results for: "sand (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)"
Corrected to: sand (NHS-SS-diazepine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
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5-Carboxyfluorescein-N-succinimidyl ester (5-FAM SE) ≥99% (by TLC) amine-reactive fluorescent dye
Supplier: Biotium
5-FAM SE is an amine-reactive form of 5-carboxyfluorescein single isomer.
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5(6)-Carboxytetramethylrhodamine succinimidyl ester [5(6)-TAMRA SE] mixture of isomers fluorescent dye, Pierce™
Supplier: Thermo Scientific
Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
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N-Succinimidyl 6-Biotinamidohexanoate ≥97.0% (by HPLC)
Supplier: TCI America
CAS Number: 72040-63-2
MDL Number: MFCD00065502
Molecular Formula: C20H30N4O6S
Molecular Weight: 454.54
Purity/Analysis Method: >97.0% (HPLC)
Form: Crystal
Color: White
Melting point (°C): 170
Storage Temperature: <0°C
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m-PEG12-NHS ester
Supplier: TCI America
Molecular Weight: 685.76
Form: Paste
Color: White
Storage Temperature: <0°C
Molecular Formula: C30H55NO16
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DSP (Dithiobis(succinimidyl propionate)), Premium Grade, Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
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N-Succinimidyl 3-(propargyloxy)propionate
Supplier: Thermo Scientific Chemicals
Acetylene-containing reagent with NHS ester functionality for reaction with amines
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D-Biotin-N-succinimidyl ester ≥98%
Supplier: Thermo Scientific Chemicals
Powder
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Azido-PEG4-NHS ester
Supplier: Thermo Scientific Chemicals
An amine reactive reagent for azide incorporation. Contains a PEG spacer
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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester ≥98% (by TLC)
Supplier: Biotium
5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.
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SIA (N-Succinimidyl iodoacetate)
Supplier: Thermo Scientific
Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
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LC-SPDP (Succinimidyl 6-[3-(2-pyridylthio)propionamidohexanoate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
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N-Succinimidyl 4,7,10,13,16-pentaoxanonadec-18-ynoate
Supplier: Thermo Scientific Chemicals
Labeling reagents with enhanced water solubility and amine reactivity as the activated NHS ester
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SBAP (Succinimidyl 3-(bromoacetamido)propionate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.
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TSAT (Tris-(succinimidyl)aminotriacetate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce TSAT is tris-(succinimidyl) aminotriacetate, a novel trifunctional crosslinker that contains three short spacers arms linked to a central nitrogen atom, each ending in an amine-reactive NHS-ester group.
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MBS (N-Succinimidyl 3-maleimidobenzoate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).
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SPDP (N-Succinimidyl-3-(2-pyridyldithio)propionate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
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SMPB (N-Succinimidyl 4-(4-Maleimidophenyl)butyrate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).
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SMPH (Succinimidyl-6-((beta-maleimidopropionamido)hexanoate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).
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GMBS (N-Succinimidyl 4-maleimidobutyrate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).
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LC-SMCC (N-Succinimidyl 6-[[4-(N-Maleimidomethyl)cyclohexyl]carboxamido]hexanoate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).
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SPB (Succinimidyl-[4-(psoralen-8-yloxy)]butyrate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.
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SIAB (N-Succinimidyl (4-iodoacetyl)aminobenzoate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
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BMPS (3-Maleimidopropionic acid N-hydroxysuccinimide ester), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).
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DSG (Disuccinimidyl glutarate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.
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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.
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Sulfo-SMPB (Sulfosuccinimidyl 4-(4-maleimidophenyl)butyrate), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).
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EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
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EMCS (6-Maleimidohexanoic acid N-hydroxysuccinimide ester), Pierce™
Supplier: Thermo Scientific
Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
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MAL-PEG-SCM (Maleimide-PEG-succinimidyl carboxy methyl)
Supplier: G-Biosciences
Maleimide PEG succinimidyl carboxymethyl Heterobifunctional Cross Linkers.