299 Results for: "assay 11-55"

Supplier: VWR

Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.

Supplier: VWR

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: Enzo Life Sciences

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

Supplier: Rockland Immunochemical

(3,3',5,5'-Tetramethylbenzidine) 1.13 mM Ideal for many immunoblotting procedures and can dramatically improve detection limits of assays on a variety of membranes. Single Component. Stable at room temperature. Absorption Wavelength: 353 nm.

Supplier: G-Biosciences

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Supplier: Prosci

CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response (1). On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120 (2). This soluble CD4 fragment (sCD4) will work in HIV neutralization assays at levels comparable to previously published results. The minor upper band seen in the Coomassie blue-stained gel is likely to be due to the presence of uncleaved signal sequence.

Supplier: MilliporeSigma

1,2-Propanediol. CAS RN 57-55-6. Formula Weight: 76.10. Density: 1.04kg/L. Assay (GC): 98% min. Color (APHA): 10 max. 20L.

Supplier: Proteintech

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)

Supplier: Biosensis

The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Supplier: ANTIBODIES.COM LLC

This Human IL-11 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-11 in plasma, tissue homogenates and other biological fluids.

Supplier: ANTIBODIES.COM LLC

This Mouse WNT-11 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse WNT-11 in plasma, tissue homogenates and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Mouse IL-11 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-11 in serum, plasma, tissue homogenates, and other biological fluids.

Supplier: Rockland Immunochemical

Anti-Alkaline Phosphatase has been assayed against 1.0 µg of Alkaline Phosphatase (Human Intestine) by immunoblot using TMB 3,3',5,5'Tetramethylbenzidine as a substrate at room temperature.

Supplier: Thermo Scientific

Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

Supplier: ANTIBODIES.COM LLC

This Rat IL-11 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-11 in plasma, tissue homogenates and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Rat Caspase-11 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Caspase-11 in serum, plasma, tissue homogenates, and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Mouse Caspase-11 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Caspase-11 in serum, plasma, tissue homogenates, and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Human Kallikrein 11 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Kallikrein 11 in serum, plasma, and other biological fluids.

Supplier: G-Biosciences

Enzyme-Linked Immunosorbent Assay (ELISA) is a useful, highly sensitive and powerful technique for the detection of proteins, chemicals, and drugs (antigens) in biological samples, including serum, blood and urine.

Supplier: ANTIBODIES.COM LLC

Human IL-11 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-11 in serum, plasma, and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Mouse IL-11 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-11 in serum, plasma, and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Human Smooth Muscle Myosin Heavy Chain 11 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Smooth Muscle Myosin Heavy Chain 11 in serum, plasma, tissue homogenates, and other biological fluids.

Supplier: ANTIBODIES.COM LLC

Human ORP-11 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human ORP-11 in serum, plasma, and other biological fluids.

Supplier: Prosci

ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

Supplier: Transgenomic

Transgenomic’s ICEme Kit is an in vitro assay that uses multiplexed PCR (MX PCR) followed by multiplexed ICE COLD-PCR (MX-ICP) for amplification and enrichment of mutations in KRAS Exons 2, 3 and 4, NRAS Exons 2, 3 and 4, BRAF Exons 11 and 15, PIK3CA Exons 9 and 20, EGFR Exons 12, 18, 19, 20 and 21.

Supplier: Tonbo Biosciences

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from na�ve or conventional T cells which are CD25-. This clone has also been reported for depletion of Treg cells in vivo (use format suitable for functional assays).

Supplier: Tonbo Biosciences

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from na�ve or conventional T cells which are CD25-. This clone has also been reported for depletion of Treg cells in vivo (use format suitable for functional assays).

Supplier: ANTIBODIES.COM LLC

Human Smooth Muscle Myosin Heavy Chain 11 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Smooth Muscle Myosin Heavy Chain 11 in serum, plasma, and other biological fluids.

Supplier: Prosci

Carbonic Anhydrase-Related Protein 11 (CA11) is a secreted protein member of the alpha-carbonic anhydrase family

Supplier: Boster Biological Technology

Sandwich ELISA kit of Quantitative Detection for Human IL-11