891 Results for: "Diagnostic Biosystems"

Supplier: Diagnostic Biosystems

This antibody is specific to two proteins of 185 kDa and 145 kDa, identified as two isoforms of neural cell adhesion molecules (NCAM/CD56). NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma and neuroblastoma.

Supplier: Diagnostic Biosystems

This antibody reacts with a 1000 kDa protein and recognizes the peptide core of gastric mucin M1. Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric mucin M1 antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or changes in the glycosylation pattern of mucin, which expose the hidden M1 antigen.

Supplier: Diagnostic Biosystems

E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Numerous studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

Supplier: Diagnostic Biosystems

This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. The PG-M1 differs from EMB11 (CD68) because of its non-reactivity with granulocytes and their precursor cells.

Supplier: Diagnostic Biosystems

Proper tissue adhesion to the glass slides is critical in the immunostaining process. Improper adhesion of tissue sections can lead to poor quality staining or even loss of tissue sections during the process of immunostaining. White glue, albumen, chromegelatin and poly-L-lysine are used as tissue adhesives.

Supplier: Diagnostic Biosystems

The clone F7.2.38 is specific for the non-glycosylated epsilon chain of the human CD3 molecule. Clone F7.2.38 recognizes T cells in the thymus, bone marrow, peripheral lymphoid tissue and blood and is a pan T cell marker. The CD3 molecule consists of five different polypeptide chains with molecular weights ranging from 16 to 28 kDa. The CD3 antigen is first detected in early thymocytes, and its appearance probably represents one of the earliest signs of commitment to the T cell lineage.

Supplier: Diagnostic Biosystems

The progesterone receptor (PgR) is an estrogen-regulated protein. It has been proposed that expression of PgR indicates a responsive estrogen receptor (ER) pathway. A number of studies have shown that PgR determination provides supplementary information to ER, both in predicting response to endocrine therapy and estimating survival. PgR has proved superior to ER as a prognostic indicator in some studies.

Supplier: Diagnostic Biosystems

This antibody is specific to 53 kDa protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.

Supplier: Diagnostic Biosystems

This antibody reacts with an 80 kDa protein known as hCdc47. hCdc47 is a member of the MCM family, which allows DNA to replicate once per cell cycle. Expression of Cdc47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCdc47-positive cells than their normal counterparts.

Supplier: Diagnostic Biosystems

This antibody recognizes a c-erbB-2 protein, which is a receptor tyrosine kinase of the c-erbB family. It is closely related in structure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a subset of breast and other adenocarcinomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.

Supplier: Diagnostic Biosystems

This antibody stains C cells of thyroid.

Supplier: Diagnostic Biosystems

This antibody is specific to CD74. This antibody can be used for the identification of B cell lymphomas and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-Reed cells.

Supplier: Diagnostic Biosystems

This antibody reacts with the β chain of all products of the gene sub regions DP, DQ, and DR. It stains B cells, interdigitating reticulum cells, Langerhans cells and many macrophages. This antibody does not react with normal T cell and polymorphs, but stains activated T cells.

Supplier: Diagnostic Biosystems

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Supplier: Diagnostic Biosystems

The clone F7.2.38 is specific for the non-glycosylated epsilon chain of the human CD3 molecule. Clone F7.2.38 recognizes T cells in the thymus, bone marrow, peripheral lymphoid tissue and blood and is a pan T cell marker. The CD3 molecule consists of five different polypeptide chains with molecular weights ranging from 16 to 28 kDa. The CD3 antigen is first detected in early thymocytes, and its appearance probably represents one of the earliest signs of commitment to the T cell lineage.

Supplier: Diagnostic Biosystems

This antibody is specific to epithelial membrane antigen (EMA), CA 15-3, or polymorphic epithelial mucin. This antibody stains an underglycosylated MUC1 often present on carcinoma cells.

Supplier: Diagnostic Biosystems

PermaBlue Plus/AP is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing alkaline phosphatase

Supplier: Diagnostic Biosystems

The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.

Supplier: Diagnostic Biosystems

GATA3 binding protein 3 or GATA3, is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. The human GATA3 gene has been mapped to chromosome 10p15. GATA =3 expression is primarily seen in breast carcinoma and urothelial carcinoma. Anti-GATA3 can also be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Supplier: Diagnostic Biosystems

This antibody reacts with cytokeratins of 58, 56, 52, 60, 51, 48 and 68 kDa MW. This antibody is well suited for the staining of a broad spectrum of human keratins.

Supplier: Diagnostic Biosystems

This antibody reacts specifically with a 32 kDa protein similar to the CD8 molecule. This antibody stains cytotoxic/suppressor T cells and shows staining patterns similar to clustered CD8 monoclonal antibodies.

Supplier: Diagnostic Biosystems

This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER). ER content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Supplier: Diagnostic Biosystems

This antibody reacts with the 52 kDa intermediate filament protein GFAP in brain and spinal cord. It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. This antibody does not react with other intermediate filament proteins.

Supplier: Diagnostic Biosystems

This antibody reacts with a 33 to 36 kDa protein known as ERCC1 (excision repair cross complementing) polypeptide. ERCC1 is required for nucleotide excision repair of damaged DNA and is homologous to RAD10. In mammalian cells, XPG cleaves 3’ of the DNA lesion while ERCC1-XPF complex makes the 5’ incision.

Supplier: Diagnostic Biosystems

Anti-Prohibitin Antibody

Supplier: Diagnostic Biosystems

Mesothelin is a glycocylphosphotidylianositol-linked cell-surface glycoprotein, which is present on the surface of normal mesothelium and is overexpressed in many patients with epithelial ovarian cancer and malignant mesotheliomas.

Supplier: Diagnostic Biosystems

This antibody is specific to an 85 kDa protein known as XRCC1. X-ray repair cross complementing 1 (XRCC1) protein plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase β through the N-terminal half. In testis XRCC1 is expressed at high levels.

Supplier: Diagnostic Biosystems

Myeloperoxidase metabolizes most of the hydrogen peroxide generated by activated phagocytes. This antibody stains strongly with the cytoplasm of myeloid cells. The antibody detects myeloperoxidase found in myeloid cells of acute myeloid leukemia. The myeloperoxidase gene has been localized to chromosome 17.

Supplier: Diagnostic Biosystems

This antibody reacts with a 52 to 56 kDa protein known as microphthalmia (Mi). This antibody reacts with both melanocytic and non-melanocytic isoforms of Mi. Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. There are two known isoforms of Mi differing by 66 amino acids at the amino terminus.

Supplier: Diagnostic Biosystems

This antibody recognizes cytokeratin polypeptides ranging from 40 to 58 kDa, corresponding to cytokeratins 5, 6, 8,17 and 19. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts and tracheal epithelium.