Flongle is an adapter for MinION and GridION devices that enables direct, real-time DNA sequencing on smaller, single-use Flongle Flow Cells.

• The most rapid, accessible, and cost-efficient nanopore sequencing system for smaller or more frequently performed assays and experiments
• Each Flongle Flow can deliver 1–2 Gb of real-time sequencing data

The Flongle Adapter allows MinION and GridION devices to run smaller, single-use Flongle Flow Cells, typically generating 1–2 Gb of data per flow cell. Users can simply insert a Flongle Flow Cell into the Flongle Adapter, run their experiment on their MinION or GridION device and then discard the Flongle Flow Cell and replace it with another.

How flow cells work: The user adds their prepared library to the flow cell and starts their sequencing experiment. Data is accumulated over time as DNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.

As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~24 hours (under optimal conditions).

Flongle R10.4.1 Flow Cells have been tested with the following sequencing kits:
Ligation Sequencing Kit V14 (SQK-LSK114)
Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
Rapid Sequencing Kit V14 (SQK-RAD114)
Rapid Barcoding Kit 24 V14 (SQK-RBK114.24)
Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24)
Native Barcoding Kit 24 V14 (SQK-NBD114.24)
Native Barcoding Kit 96 V14 (SQK-NBD114.96)
16S Barcoding Kit 24 V14 (SQK-16S114.24)