VWR® Taq DNA polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimization.

• A thermostable recombinant DNA polymerase
• High product yield
• dUTP incorporation possible
• Processes up to 5 kb
• Leaves a 3'dA overhang

Taq DNA polymerase concentration: 5 Units/μl

10X Key Buffer: Tris-HCl pH 8.5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8.3; KCl, 15 mM MgCl₂, 1% Tween®20
10X Key Buffer (Mg²⁺ free): Tris-HCl pH 8.5; (NH₄)₂SO₂, 1% Tween® 20
10X Extra Buffer (Mg²⁺ free): Tris-HCl pH 8.3; KCl, 1% Tween®20

Taq DNA polymerase is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). It leaves an A' overhang, which makes the enzyme ideal for TA cloning.

Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high-throughput testing.

Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Optional: Store at +4 °C for up to 6 months.

Additional MgCl₂ is included with each kit to enable optimization.

Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimizing the need for optimization of the Mg²⁺ concentration or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimization of primer annealing temperatures and Mg²⁺ concentrations may be required.

Taq DNA polymerase is also available in a glycerol-free format for automation and lyophilization.