To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Description:Monarch® HMW DNA extraction kits for tissue
- Size:5 preps
- Cat. no.:CA76470-750
Specifications
About this item
The Monarch® HMW DNA extraction kit for tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian.
- Isolate DNA into the Mb size range from soft organ tissues and bacteria
- Fast, user-friendly workflows (90 minutes for tissues and bacteria)
- Obtain excellent yields of highly-pure and intact DNA; ideal for long read sequencing
- Includes RNase A and Proteinase K, as well as microtube pestles for convenient sample homogenization
The optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to ≥500 kb for the standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For tissue and bacteria, the processing time is 90 min. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing.
Supplier: VWR International
$194.14 to $694.12
Supplier: VWR International
$377.44 to $992.90