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Specifications
- Description:AccuStart™ II PCR SuperMix
- Size:100 rxn (of 20 µl rxn)
- Cat. no.:CA76047-072
Specifications
About this item
AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template.
- High yield, high sensitivity
- Precise amplification - hot-start technology ensures specific and efficient primer extension
- Superior reproducibility and precision
- Convenient reaction assembly at room temperature
AccuStart II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Contents:
- 2X reaction buffer containing 3 mM MgCl2
- 4 mM each dNTP (dATP, dCTP, dGTP, dTTP)
- AccuStart II Taq DNA Polymerase and stabilizers.
Storage conditions:
AccuStart II PCR SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C. For convenience, it may be stored unfrozen at +2 to +8°C for up to 12 months. Repeated freezing and thawing does not impair product performance.
Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.