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Specifications
- Size:20000 units
- Enzyme name:Hi-T4 DNA Ligase
- Concentration:400000 units/ml
- Cat. no.:CA76407-354
Specifications
About this item
A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.
- Hi-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved thermostability
- Capable of joining blunt end and cohesive end termini
- Capable of repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids
- Supplied with both T4 DNA Ligase buffer and StickTogether DNA Ligase buffer
Hi-T4 DNA Ligase will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids at temperatures as high as 50 °C.
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