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Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Total DNA
- Sample type:Immunoprecipitates
- Target molecule:DNA
- Description:ChIP DNA Clean Concentrator Kit, Uncapped Column
- Size:50 preps
- Description:ChIP DNA Clean Concentrator Kit
- Cat. no.:77001-700
Specifications
About this item
The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high-quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP.
- Two minute DNA clean-up from any step in a standard ChIP protocol.
- DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.
These kits may be applied to any routine ChIP procedure to determine DNA concentration of samples that have undergone reverse cross-linking following DNA shearing.
Specifications
Elution Volume: ≥ 6 µl
Processing Time: 2 minutes
Recovery: 70-90% for DNA 50 bp - 10 kb, 70% for DNA > 10 kb
Size Range: 50 bp - 23 kb
Storage: All components should be stored at room temperature.
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