Purified, non-methylated and methylated human DNA for use as negative and positive control in methylated detection applications.

• Each standard is provided with primer set to amplify a fragment of DNA after bisulfite conversion

The Human Methylated and Non-methylated DNA Set consists of two control DNAs (a methylated human DNA standard and a non-methylated human DNA standard) together with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation™ family of products to assess the efficiency of bisulfite-mediated conversion of DNA.

The non-methylated human DNA is purified from the HCT116 DKO (double knock-out) cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The DNA derived from HCT116 DKO cells has a low level of DNA methylation (< 5%) and therefore can be used as a negative control for DNA methylation analysis (see below). The methylated human DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by CpG Methylase and can be used as a positive control for DNA methylation analysis.

The Human WGA Methylated & Non-methylated DNA Set is ideal for studying loci with ultralow methylation levels. This set is generated from HCT116 DKO DNA using whole-genome amplification (WGA) technology.