Monarch DNA Cleanup Kits, available for both gel extraction and reaction cleanup, are optimized for maximum performance and minimal environmental impact.

• Elute in as little as 6 μl
• Prevent buffer retention and salt carry-over with optimized column design
• Save time with fast, user-friendly protocols
• No need to monitor pH or add isopropanol
• Buffers and columns available separately
• Significantly less plastic used when compared with other kits
• Responsibly-sourced and recyclable packaging

These kits rapidly and reliably purify up to 5 μg of concentrated, highquality DNA and utilize a bind/wash/elute workflow with minimal incubation and spin times. The columns provided with each kit are specifically designed to eliminate buffer retention and the risk of carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Optimized buffer systems eliminate the need for monitoring and adjusting pH

The Monarch DNA Gel Extraction Kit can be used to quickly purify DNA from agarose gels. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step.
The Monarch PCR & DNA Cleanup Kit (5 μg) can be used to purify DNA from a variety of enzymatic reactions. The DNA Wash Buffer provided ensures enzymes, short primers (≤ 25 nt), detergents and other low-molecular weight reaction components are removed.
Both kits use significantly less plastic and responsibly-sourced, recyclable packaging, so you can feel good about choosing Monarch for your DNA purification.

Specifications
• Binding Capacity: up to 5 μg
• DNA Size Range: ~50 bp to 25 kb
• Typical Recovery: DNA (50 bp to 10 kb): 70–90% DNA (11–25 kb): 50–70%
• Elution Volume: ≥ 6 μl
• Purity: A260/A280 ≥ 1.8
• Protocol Time: Gel Extraction: 10 min of spin and incubation time PCR & DNA Cleanup: 5 min of spin and incubation time
• Compatible Downstream Applications: ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing