The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends.

• Optimized for use with the NEBNext Ultra II Ligation Module
• Compatible with some Oxford Nanopore Technologies workflows

The module is optimized for use with the NEBNext Ultra II End Ligation Module (NEB #E7595), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This module is compatible with Illumina workflows and with some Oxford Nanopore MinION™ workflows. For functional validation, each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.