The SF cell line 4D-Nucleofector® X kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µl 16-well Nucleocuvette® strip format when working with the 4D-Nucleofector® X unit.

• High transfection efficiencies in difficult-to-transfect cell lines
• Cells maintain functionality post transfection
• Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
• Transferability to 4D-Nucleofector® LV Unit

This kit has been determined to be optimal for transfecting following cell types:
293-F (FreeStyle), 32D
A-20, A2058, A375, A431, A549, AGS, ARPE-19
BetaTC/6, BxPC-3
C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
EL4
FDC-P1
H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
IMR-32
J774A.1
K-562
KG-1
L-428, LNCaP
MDA-MB-453, MDBK, Molt-4, MV-4-11
Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
P19, P815, PC-12, PC-3
Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
T2, T84, TF-1
U266B1, U-937
Vero

Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
Medium throughput applications.
Optimization of transfection conditions e.g. substrate titrations.

The kit is available in different variants for either 12 or 24 reactions.