Specifications
- Description:SE cell line 4D-Nucleofector® X kit S
- Cat. no.:CA10064-150
- No. of Reactions:32
- Supplier No.:V4XC-1032
Specifications
About this item
The SE Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.
- Benefits:
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
This kit has been determined to be optimal for transfecting following cell types:
3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D, T84
U2OS, U-87 MG
WI-38
Applications:
Transfection of low cell numbers in the range of 5×10⁴ to 1×10⁶ cells.
Medium throughput applications.
Optimization of transfection conditions e.g. substrate titrations.
The kit is available in different variants for either 12 or 24 reactions.
1×0.675 ml SF cell line Nucleofector® solution.
1×0.15 ml Supplement 1.
50 µg pmaxGFP® vector (1 μg/μl in 10 mM Tris pH 8.0).
2×16-well Nucleocuvette® strips (20 μl).
Supplier: Eppendorf
$224.54