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Specifications
- Species:Human
- Size:1000 units
- Storage conditions:Store at −20 °C
- Enzyme name:HRV 3C protease solution kit
- Enzyme activity:2 units/µl, 10000 units
- Cat. no.:CAPI88946
- Supplier No.:88946
Specifications
About this item
Thermo Scientific Pierce Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
- Highly Specific with no Off-Target Cleavage Even at High Enzyme/Substrate Concentration
- Allows Cleavage Reaction to Take Place at 4°C Without Affecting the Functionality of Purified Protein
- Can be used Either Following Affinity Purification or While Fusion Proteins are Bound to Glutathione or a Tag-Specific Resin
- Dual-Tagged Enzyme is Compatible with Various Columns and Buffers
Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-⊥-Gly-Pro and cleaves after the glutamine residue
Alternative names: Human Rhinovirus 3C
Source: Escherichia coli (E.coli)
Molecular weight: 47.8kD
Form: Liquid
Protease type: Cysteine
Optimal reaction conditions: 50 mM Tris-HCl, 150 mM NaCl, pH 8.0
The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
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