To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Description:Gaussia luciferase flash assay kit
- Size:100 reactions
- Cat. no.:CAPI16158
- Supplier No.:16158
Specifications
About this item
The Thermo Scientific Pierce Gaussia luciferase flash assay kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
- 1000 times greater sensitivity allows utilization of smaller numbers of cells
- Highest sensitivity assays result in decreased reagent consumption
- Secreted luciferase allows real-time assays and kinetic studies without destroying cells
- Assays using secreted luciferases require minimal sample handling
- Amenable to automation
- Convenient and safe - no radioactivity required
Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity.
Kit may be stored at –80 ˚C, or individually at indicated temperatures.
Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use our Gaussia-Dura Luc Vectors.)
The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.
Supplier: BDH
729,52 $